PROTOCOL TO MEASURE THE MEMBRANE TENSION AND BENDING MODULUS OF CELLS USING OPTICAL TWEEZERS AND SCANNING ELECTRON MICROSCOPY

Protocol to measure the membrane tension and bending modulus of cells using optical tweezers and scanning electron microscopy

Summary: The elastic properties of cell membranes, particularly the membrane tension and Circuit Board With Capacitor bending modulus, are known to be key regulators of cellular functions.Here, we present a correlative and integrated tool based on optical tweezers and scanning electron microscopy to accurately determine these properties in a variet

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BEMO: A Parsimonious Big Data Mining Methodology

The Problem: Standardized processes are often followed to systematically conduct data mining projects.However while current models provide good descriptions, they are in need of updates given current Big Data challenges.Current data mining methods do not meet all requirements of businesses, in addition current methods are difficult to remember and

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Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination

Summary: Meiotic recombination is critical for genetic exchange and generation of chiasmata that ensures faithful chromosome segregation during meiosis I.Meiotic recombination is initiated by DNA double-strand break (DSB) followed by multiple processes of DNA repair.The exact mechanisms for how recombinases localize to DSB remain elusive.Here, we s

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